锁核酸Locked nucleic acidLNA)是一种经过修饰的RNA,LNA中一部分核糖上的2'与4'碳连结在一起。一般可见于A-DNARNA

这类核酸可以增加引子探针(probe)的融解温度(Tm值),加强这些实验用物质的稳定性,可应用在即时聚合酶连锁反应(real-time PCR)等技术中。

参考文献

  • Kaur, H.; Arora, A.; Wengel, J.; Maiti, S. Thermodynamic, Counterion, and Hydration Effects for the Incorporation of Locked Nucleic Acid Nucleotides into DNA Duplexes. Biochemistry. 2006, 45 (23): 7347–55. doi:10.1021/bi060307w.

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