15-hydroxyprostaglandin-D dehydrogenase (NADP+)

In enzymology, a 15-hydroxyprostaglandin-D dehydrogenase (NADP⁺) (EC 1.1.1.196) is an enzyme that catalyzes the chemical reaction

(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADP⁺ ${\displaystyle \rightleftharpoons }$ (5Z,13E)-9alpha-hydroxy-11,15-dioxoprosta-5,13-dienoate + NADPH + H⁺

15-hydroxyprostaglandin-D dehydrogenase (NADP+)
Identifiers
EC no.	1.1.1.196
CAS no.	84399-95-1
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Thus, the two substrates of this enzyme are (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate and NADP⁺, whereas its 3 products are (5Z,13E)-9alpha-hydroxy-11,15-dioxoprosta-5,13-dienoate, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate:NADP⁺ 15-oxidoreductase. Other names in common use include prostaglandin-D 15-dehydrogenase (NADP⁺), dehydrogenase, prostaglandin D2, NADP⁺-PGD2 dehydrogenase, dehydrogenase, 15-hydroxyprostaglandin (nicotinamide adenine, dinucleotide phosphate), 15-hydroxy PGD2 dehydrogenase, 15-hydroxyprostaglandin dehydrogenase (NADP⁺), NADP⁺-dependent 15-hydroxyprostaglandin dehydrogenase, prostaglandin D2 dehydrogenase, NADP⁺-linked 15-hydroxyprostaglandin dehydrogenase, NADP⁺-specific 15-hydroxyprostaglandin dehydrogenase, NADP⁺-linked prostaglandin D2 dehydrogenase, and 15-hydroxyprostaglandin-D dehydrogenase (NADP⁺). This enzyme participates in arachidonic acid metabolism.

References

• Watanabe K, Shimizu T, Iguchi S, Wakatsuka H, Hayashi M, Hayaishi O (1980). "An NADP-linked prostaglandin D dehydrogenase in swine brain". J. Biol. Chem. 255 (5): 1779–82. PMID 7354056.