Dolichyl-phosphate beta-D-mannosyltransferase

In enzymology, a dolichyl-phosphate beta-D-mannosyltransferase (EC 2.4.1.83) is an enzyme that catalyzes the chemical reaction

GDP-mannose + dolichyl phosphate ${\displaystyle \rightleftharpoons }$ GDP + dolichyl D-mannosyl phosphate

dolichyl-phosphate beta-D-mannosyltransferase
Identifiers
EC no.	2.4.1.83
CAS no.	62213-44-9
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Thus, the two substrates of this enzyme are GDP-mannose and dolichyl phosphate, whereas its two products are GDP and dolichyl D-mannosyl phosphate.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is GDP-mannose:dolichyl-phosphate beta-D-mannosyltransferase. Other names in common use include GDP-Man:DolP mannosyltransferase, dolichyl mannosyl phosphate synthase, dolichyl-phospho-mannose synthase, GDP-mannose:dolichyl-phosphate mannosyltransferase, guanosine diphosphomannose-dolichol phosphate mannosyltransferase, dolichol phosphate mannose synthase, dolichyl phosphate mannosyltransferase, dolichyl-phosphate mannose synthase, GDP-mannose-dolichol phosphate mannosyltransferase, GDP-mannose-dolichylmonophosphate mannosyltransferase, mannosylphosphodolichol synthase, and mannosylphosphoryldolichol synthase. This enzyme participates in n-glycan biosynthesis.

References

• Babczinski P, Haselbeck A, Tanner W (1980). "Yeast mannosyl transferases requiring dolichyl phosphate and dolichyl phosphate mannose as substrate. Partial purification and characterization of the solubilized enzyme". Eur. J. Biochem. 105 (3): 509–15. doi:10.1111/j.1432-1033.1980.tb04526.x. PMID 6989607.
• Bretthauer RK, Wu S, Irwin WE (1973). "Enzymatic transfer of mannose from guanosine diphosphate mannose to dolichol phosphate in yeast (Hansenula holstii). A possible step in mannan synthesis". Biochim. Biophys. Acta. 304 (3): 736–47. doi:10.1016/0304-4165(73)90220-1. PMID 4726855.
• Haselbeck A (1989). "Purification of GDP mannose:dolichyl-phosphate O-beta-D-mannosyltransferase from Saccharomyces cerevisiae". Eur. J. Biochem. 181 (3): 663–8. doi:10.1111/j.1432-1033.1989.tb14774.x. PMID 2659345.
• Palamarczyk G, Lehle L, Mankowski T, Chojnacki T, Tanner W (1980). "Specificity of solubilized yeast glycosyl transferases for polyprenyl derivatives". Eur. J. Biochem. 105 (3): 517–23. doi:10.1111/j.1432-1033.1980.tb04527.x. PMID 6445267.
• Richards JB, Hemming FW (1972). "The transfer of mannose from guanosine diphosphate mannose to dolichol phosphate and protein by pig liver endoplasmic reticulum". Biochem. J. 130 (1): 77–93. PMC 1174303. PMID 4655455.