Coenzyme F420 hydrogenase

In enzymology, a coenzyme F420 hydrogenase (EC 1.12.98.1) is an enzyme that catalyzes the chemical reaction

H₂ + coenzyme F₄₂₀ ${\displaystyle \rightleftharpoons }$ reduced coenzyme F₄₂₀

coenzyme F420 hydrogenase
Identifiers
EC no.	1.12.98.1
CAS no.	9027-05-8
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Thus, the two substrates of this enzyme are H₂ and coenzyme F420, whereas its product is reduced coenzyme F420.

This enzyme belongs to the family of oxidoreductases, specifically those acting on hydrogen as donor with other, known, acceptors. The systematic name of this enzyme class is hydrogen:coenzyme F420 oxidoreductase. Other names in common use include 8-hydroxy-5-deazaflavin-reducing hydrogenase, F420-reducing hydrogenase, and coenzyme F420-dependent hydrogenase. This enzyme participates in folate biosynthesis and is a critical part of energy conservation in some methanogens such as Methanosarcina barkeri. It has 3 cofactors: iron, nickel, and deazaflavin.

References

• Adams MWW, Mortenson LE, Chen J-S (1981). "Hydrogenase". Biochim. Biophys. Acta. 594: 105–176. doi:10.1016/0304-4173(80)90007-5.
• Yamazaki S (1982). "A selenium-containing hydrogenase from Methanococcus vannielii Identification of the selenium moiety as a selenocysteine residue". J. Biol. Chem. 257 (14): 7926–9. PMID 6211447.
• Fox JA, Livingston DJ, Orme-Johnson WH, Walsh CT (1987). "8-Hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization". Biochemistry. 26 (14): 4219–27. doi:10.1021/bi00388a007. PMID 3663585.
• Muth E, Morschel E, Klein A (1987). "Purification and characterization of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from the archaebacterium Methanococcus voltae". Eur. J. Biochem. 169 (3): 571–7. doi:10.1111/j.1432-1033.1987.tb13647.x. PMID 3121317.
• Baron SF, Ferry JG (1989). "Purification and properties of the membrane-associated coenzyme F420-reducing hydrogenase from Methanobacterium formicicum". J. Bacteriol. 171 (7): 3846–53. PMC 210134. PMID 2738024.