Tetrahydroberberine oxidase
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(S)-Tetrahydroberberine oxidase (EC 1.3.3.8) is an enzyme that catalyzes the final transformation in the biosynthesis of berberine, a quaternary benzylisoquinoline alkaloid of the protoberberine structural subgroup.[1][2] This reaction pathway catalyzes the four-electron oxidation of (S)-tetrahydroberberine (also known as (S)-canadine) in the presence of oxygen to produce berberine and hydrogen peroxide as products.
tetrahydroberberine oxidase | |||||||||
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Identifiers | |||||||||
EC no. | 1.3.3.8 | ||||||||
CAS no. | 114705-00-9 | ||||||||
Databases | |||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB structures | RCSB PDB PDBe PDBsum | ||||||||
Gene Ontology | AmiGO / QuickGO | ||||||||
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(S)-Tetrahydroberberine + 2 O2 ⇌ Berberine + 2 H2O2
This enzyme belongs to the family known as oxidoreductases, in this instance the CH-CH moiety acts as the electron donor with oxygen acting as the electron acceptor. The systematic name of this enzyme is (S)-tetrahydroberberine:oxygen oxidoreductase; but it is also known as (S)-THB oxidase, tetrahydroberberine oxidase, and decreasingly, as (S)-tetrahydroprotoberberine oxidase.[3][4]