Formate–tetrahydrofolate ligase
From Wikipedia, the free encyclopedia
In enzymology, a formate—tetrahydrofolate ligase (EC 6.3.4.3) is an enzyme that catalyzes the chemical reaction
- ATP + formate + tetrahydrofolate ADP + phosphate + 10-formyltetrahydrofolate
Formate—tetrahydrofolate ligase | |||||||||
---|---|---|---|---|---|---|---|---|---|
Identifiers | |||||||||
EC no. | 6.3.4.3 | ||||||||
CAS no. | 9023-66-9 | ||||||||
Databases | |||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB structures | RCSB PDB PDBe PDBsum | ||||||||
Gene Ontology | AmiGO / QuickGO | ||||||||
|
Formate—tetrahydrofolate ligase | |||||||||
---|---|---|---|---|---|---|---|---|---|
Identifiers | |||||||||
Symbol | FTHFS | ||||||||
Pfam | PF01268 | ||||||||
Pfam clan | CL0023 | ||||||||
InterPro | IPR000559 | ||||||||
PROSITE | PDOC00595 | ||||||||
SCOP2 | 1fpm / SCOPe / SUPFAM | ||||||||
|
The 3 substrates of this enzyme are ATP, formate, and tetrahydrofolate, whereas its 3 products are ADP, phosphate, and 10-formyltetrahydrofolate.
This enzyme belongs to the family of ligases, specifically those forming generic carbon-nitrogen bonds. This enzyme participates in glyoxylate and dicarboxylate metabolism and one carbon pool by folate.
In eukaryotes the FTHFS activity is expressed by a multifunctional enzyme, C-1-tetrahydrofolate synthase (C1-THF synthase), which also catalyses the dehydrogenase and cyclohydrolase activities. Two forms of C1-THF syntheses are known, one is located in the mitochondrial matrix, while the second one is cytoplasmic.[1] In both forms the FTHFS domain consists of about 600 amino acid residues and is located in the C-terminal section of C1-THF synthase. In prokaryotes FTHFS activity is expressed by a monofunctional homotetrameric enzyme of about 560 amino acid residues.[2]