Lightsheetfluorescencemicroscopy (LSFM) is a fluorescencemicroscopy technique with an intermediate-to-high optical resolution, but good optical sectioning
intrinsic fluorescence of the protein or by using transmission microscopy. Both methods require an ultraviolet microscope as proteins absorbs light at 280 nm
Structured illumination lightsheetmicroscopy (SI-LSM) is an optical imaging technique used for achieving volumetric imaging with high temporal and spatial