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Available structures
PDBOrtholog search: PDBe RCSB
AliasesPUF60, FIR, RoBPI, SIAHBP1, VRJS, poly(U) binding splicing factor 60KDa, poly(U) binding splicing factor 60
External IDsOMIM: 604819 MGI: 1915209 HomoloGene: 8614 GeneCards: PUF60
Gene location (Human)
Chr.Chromosome 8 (human)[1]
Band8q24.3Start143,816,344 bp[1]
End143,829,352 bp[1]
RNA expression pattern

More reference expression data
RefSeq (mRNA)


RefSeq (protein)


Location (UCSC)Chr 8: 143.82 – 143.83 MbChr 15: 76.07 – 76.08 Mb
PubMed search[3][4]
View/Edit HumanView/Edit Mouse

Poly(U)-binding-splicing factor PUF60 is a protein that in humans is encoded by the PUF60 gene.[5][6][7][8]

The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.[8]


PUF60 has been shown to interact with U2AF2.[9][10]


  1. ^ a b c ENSG00000274081 GRCh38: Ensembl release 89: ENSG00000179950, ENSG00000274081 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000002524 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Bouffard P, Barbar E, Brière F, Boire G (January 2000). "Interaction cloning and characterization of RoBPI, a novel protein binding to human Ro ribonucleoproteins". RNA. 6 (1): 66–78. doi:10.1017/S1355838200990277. PMC 1369894. PMID 10668799.
  6. ^ Liu J, He L, Collins I, Ge H, Libutti D, Li J, Egly JM, Levens D (February 2000). "The FBP interacting repressor targets TFIIH to inhibit activated transcription". Molecular Cell. 5 (2): 331–41. doi:10.1016/S1097-2765(00)80428-1. PMID 10882074.
  7. ^ Hastings ML, Allemand E, Duelli DM, Myers MP, Krainer AR (June 2007). Valcarcel J (ed.). "Control of pre-mRNA splicing by the general splicing factors PUF60 and U2AF(65)". PLOS ONE. 2 (6): e538. Bibcode:2007PLoSO...2..538H. doi:10.1371/journal.pone.0000538. PMC 1888729. PMID 17579712. open access
  8. ^ a b "Entrez Gene: SIAHBP1 fuse-binding protein-interacting repressor".
  9. ^ Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514.
  10. ^ Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.

Further reading

  • Page-McCaw PS, Amonlirdviman K, Sharp PA (December 1999). "PUF60: a novel U2AF65-related splicing activity". RNA. 5 (12): 1548–60. doi:10.1017/S1355838299991938. PMC 1369877. PMID 10606266.
  • Poleev A, Hartmann A, Stamm S (July 2000). "A trans-acting factor, isolated by the three-hybrid system, that influences alternative splicing of the amyloid precursor protein minigene". European Journal of Biochemistry. 267 (13): 4002–10. doi:10.1046/j.1432-1327.2000.01431.x. PMID 10866799.
  • Liu J, Akoulitchev S, Weber A, Ge H, Chuikov S, Libutti D, Wang XW, Conaway JW, Harris CC, Conaway RC, Reinberg D, Levens D (February 2001). "Defective interplay of activators and repressors with TFIH in xeroderma pigmentosum". Cell. 104 (3): 353–63. doi:10.1016/S0092-8674(01)00223-9. PMID 11239393.
  • Sandrock B, Egly JM (September 2001). "A yeast four-hybrid system identifies Cdk-activating kinase as a regulator of the XPD helicase, a subunit of transcription factor IIH". The Journal of Biological Chemistry. 276 (38): 35328–33. doi:10.1074/jbc.M105570200. PMID 11445587.
  • Jin J, Smith FD, Stark C, Wells CD, Fawcett JP, Kulkarni S, Metalnikov P, O'Donnell P, Taylor P, Taylor L, Zougman A, Woodgett JR, Langeberg LK, Scott JD, Pawson T (August 2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Current Biology. 14 (16): 1436–50. doi:10.1016/j.cub.2004.07.051. PMID 15324660.
  • Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE (September 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. PMID 16169070.
  • Liu J, Kouzine F, Nie Z, Chung HJ, Elisha-Feil Z, Weber A, Zhao K, Levens D (May 2006). "The FUSE/FBP/FIR/TFIIH system is a molecular machine programming a pulse of c-myc expression". The EMBO Journal. 25 (10): 2119–30. doi:10.1038/sj.emboj.7601101. PMC 1462968. PMID 16628215.
  • Lim J, Hao T, Shaw C, Patel AJ, Szabó G, Rual JF, Fisk CJ, Li N, Smolyar A, Hill DE, Barabási AL, Vidal M, Zoghbi HY (May 2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569.
  • PDBe-KB provides an overview of all the structure information available in the PDB for Human Poly(U)-binding-splicing factor PUF60

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