User:Lamals/sandbox/STED microscopy
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Stimulated emission depletion (STED) microscopy is a far field super-resolution microscopy technique. It was developed by Stefan W. Hell and Jan Wichmann in 1994, and was first experimentally demonstrated by Hell and Thomas Klar in 1999.[1] Hell was awarded the Nobel Prize in Chemistry in 2014 for its development.
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STED microscopy was developed to bypass the diffraction limit of light microscopy to increase resolution of florescence images. STED exploits a non-linear response of fluorophores, minimizing the illuminating area of the focal point through selective deactivation of florescence, to achieve improved resolution below the diffraction limit. This technique differs from other super resolution microscopy techniques such as Photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) as these methods use mathematical models to reconstruct a sub diffraction limit from many sets of diffraction limited images.