2'-Hydroxyisoflavone reductase

In enzymology, a 2'-hydroxyisoflavone reductase (EC 1.3.1.45) is an enzyme that catalyzes the chemical reaction

vestitone + NADP⁺ ${\displaystyle \rightleftharpoons }$ 2'-hydroxyformononetin + NADPH + H⁺

2'-hydroxyisoflavone reductase
Identifiers
EC no.	1.3.1.45
CAS no.	128449-69-4
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Thus, the two substrates of this enzyme are vestitone and NADP⁺, whereas its 3 products are 2'-hydroxyformononetin, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is vestitone:NADP+ oxidoreductase. Other names in common use include NADPH:2'-hydroxyisoflavone oxidoreductase, isoflavone reductase, and 2',7-dihydroxy-4',5'-methylenedioxyisoflavone reductase.