2-Hydroxy-1,4-benzoxazin-3-one monooxygenase

2-Hydroxy-1,4-benzoxazin-3-one monooxygenase (EC 1.14.13.140, BX5 (gene), CYP71C3 (gene)) is an enzyme with systematic name 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one,NAD(P)H:oxygen oxidoreductase (N-hydroxylating).^[1][2] This enzyme catalyses the following chemical reaction

2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + NAD(P)H + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + NAD(P)+ + H₂O

2-Hydroxy-1,4-benzoxazin-3-one monooxygenase
Identifiers
EC no.	1.14.13.140
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

2-Hydroxy-1,4-benzoxazin-3-one monooxygenase is involved in the biosynthesis of protective and allelopathic benzoxazinoids in some plants.