| Rank | Heat | Nation | Athletes | Time | Notes |
| 1 | 3 |  United States | Symone Mason, Shae Anderson, Jan'Taijah Ford, Arria Minor | 3:33.11 | Q, WJL |
| 2 | 1 |  Jamaica | Janielle Josephs, Stacey-Ann Williams, Chrissani May, Calisha Taylor | 3:34.22 | Q, WJL |
| 3 | 2 |  Australia | Ella Connolly, Cara Jardine, Jemima Russell, Carley Thomas | 3:35.48 | Q, SB |
| 4 | 1 |  Italy | Camilla Pitzalis, Eloisa Coiro, Chiara Gherardi, Elisabetta Vandi | 3:35.86 | Q, NJR |
| 5 | 2 |  Canada | Xahria Santiago, Aurora Rynda, Sharelle Samuel, Ashlan Best | 3:36.14 | Q, SB |
| 6 | 3 |  Dominican Republic | Milagros Durán, Lilian Reyes, Maria Fernanda Matos, Fiordaliza Cofil | 3:36.22(.214) | Q, NJR |
| 7 | 1 |  Brazil | Marlene Santos, Tiffani Silva, Chayenne Da Silva, Leticia Lima | 3:36.22(.215) | q, AJR |
| 8 | 3 |  Germany | Anna-Maria Hofmann, Lisa Sophie Hartmann, Johanna Berrens, Laura Kaufmann | 3:36.50 | q |
| 9 | 2 |  Japan | Ayano Shiomi, Shuri Aono, Moeka Sekimoto, Ayaka Kawata | 3:36.70 | SB |
| 10 | 1 |  Poland | Natalia Widawska, Natalia Wosztyl, Weronika Bartnowska, Karolina Łozowska | 3:38.23 | SB |
| 11 | 1 |  Hungary | Janka Molnár, Hanna Répássy, Mira Koszegi, Sára Mátó | 3:38.49 | SB |
| 12 | 2 |  India | V Subha, Jisna Mathew, Ritika, Hima Das | 3:39.10 | NJR |
| 13 | 3 |  Ethiopia | Mahilet Fikre, Diribe Welteji, Zinash Tesfaye, Frehiywot Wondie | 3:39.29 | NJR |
| 14 | 3 |  Finland | Nea Mattila, Mette Baas, Wilma Lassfolk, Viivi Lehikoinen | 3:39.58 | SB |
| 15 | 1 |  China | Tianlu Lan, Yu Zhou, Jiadie Mo, Nuo Liang | 3:42.04 | SB |
| 16 | 3 |  Slovakia | Emma Zapletalová, Mária Šimlovicová, Sophia Zápotocná, Miriam Cidoríková | 3:44.05 | NJR |
| 17 | 2 |  Ukraine | Maryana Shostak, Olena Radyuk, Tetyana Kaysen, Tetyana Bezshyyko | 3:44.74 | SB |
| 18 | 3 |  South Africa | Liza Kellerman, Marlie Viljoen, Nicola Gibbon, Zeney Van Der Walt | 3:50.39 | |
| 19 | 2 |  Sri Lanka | Sandumini Bandara, Shyamali Kumarasinghe, Romeshi Ishara Attidiya, Amasha De Silva | 3:51.02 | |
United States
Germany
