Rank | Heat | Nation | Athletes | Time | Notes |
1 | 1 | Great Britain | Chijindu Ujah, Harry Aikines-Aryeetey, Adam Gemili, Nethaneel Mitchell-Blake | 38.11 | Q, WL, *WC |
2 | 1 | Brazil | Rodrigo do Nascimento, Jorge Vides, Derick Silva, Paulo André de Oliveira | 38.22 | Q, SB, *WC |
3 | 2 | Italy | Eseosa Desalu, Marcell Jacobs, Davide Manenti, Filippo Tortu | 38.29 | Q, SB, *WC |
4 | 3 | United States | Mike Rodgers, Justin Gatlin, Isiah Young, Cameron Burrell | 38.34 | Q, SB, *WC |
5 | 2 | China | Wu Zhiqiang, Xie Zhenye, Su Bingtian, Liang Jinsheng | 38.46 | Q, SB, *WC |
6 | 2 | Turkey | Emre Zafer Barnes, Jak Ali Harvey, İzzet Safer, Ramil Guliyev | 38.47 | q, SB, *WC |
7 | 3 | France | Mouhamadou Fall, Jimmy Vicaut, Mickaël-Meba Zézé, Stuart Dutamby | 38.51 | Q, SB, *WC |
8 | 1 | Jamaica | Nesta Carter, Julian Forte, Rasheed Dwyer, Tyquendo Tracey | 38.51 | q, SB, *WC |
9 | 1 | South Africa | Emile Erasmus, Simon Magakwe, Anaso Jobodwana, Akani Simbine | 38.66 | SB, *WC |
10 | 2 | Netherlands | Christopher Garia, Churandy Martina, Hensley Paulina, Taymir Burnet | 38.67 | SB, *WC |
11 | 3 | Canada | Gavin Smellie, Aaron Brown, Brendon Rodney, Andre De Grasse | 38.76 | SB |
12 | 2 | Czech Republic | Zdeněk Stromšík, Jan Veleba, Jan Jirka, Dominik Záleský | 38.77 | NR |
13 | 2 | Thailand | Ruttanapon Sowan [fr], Nutthapong Veeravongratanas, Jirapong Meenapra, Siripol Punpa [fr] | 38.82 | |
14 | 2 | Ukraine | Oleksandr Sokolov, Emil Ibrahimov, Volodymyr Suprun, Serhiy Smelyk | 38.84 | SB |
15 | 3 | Chinese Taipei | Wei Tai-sheng, Wang Wei-hsu, Yang Chun-han, Cheng Po-yu | 38.89 | SB |
16 | 1 | Germany | Kevin Kranz, Patrick Domogala [de], Julian Reus, Robin Erewa | 38.91 | SB |
17 | 1 | Australia | Trae Williams, Alex Hartmann, Zach Holdsworth, Jake Doran [fr] | 39.05 | |
18 | 3 | Dominican Republic | Christopher Valdez, Yohandris Andújar, Mayovanex de Óleo, Yancarlos Martínez | 39.12 | |
19 | 1 | Indonesia | Mochammad Bisma Diwa Abina, Lalu Muhammad Zohri, Eko Rimbawan, Bayu Kertanegara [fr] | 39.39 | SB |
20 | 3 | Finland | Otto Ahlfors, Ville Myllymäki, Oskari Lehtonen, Samuel Purola [fr] | 39.59 | SB |
21 | 1 | Venezuela | Alberto Aguilar, Rafael Vázquez, Alexis Nieves, Abdel Kalil | 39.76 | SB |
| 1 | Poland | Remigiusz Olszewski, Przemysław Słowikowski, Karol Kwiatkowski, Dominik Kopeć | DNF | |
| 2 | Zimbabwe | Dicksson Kamungeremu, Tatenda Tsumba, Itayi Vambe, Ngoni Makusha | DQ | R163.3(b)[3] |
| 3 | Japan | Shuhei Tada, Ryota Yamagata, Yuki Koike, Yoshihide Kiryu | DQ | 170.6(a)[4] |
| 3 | Nigeria | Enoch Adegoke, Emmanuel Arowolo, Ogho-Oghene Egwero, Usheoritse Itsekiri | DQ | 170.6(a)[5] |