3-hydroxybenzoate 4-monooxygenase

In enzymology, a 3-hydroxybenzoate 4-monooxygenase (EC 1.14.13.23) is an enzyme that catalyzes the chemical reaction

3-hydroxybenzoate + NADPH + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ 3,4-dihydroxybenzoate + NADP⁺ + H₂O

3-hydroxybenzoate 4-monooxygenase
Identifiers
EC no.	1.14.13.23
CAS no.	37256-76-1
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

The 4 substrates of this enzyme are 3-hydroxybenzoate, NADPH, H⁺, and O₂, whereas its 3 products are 3,4-dihydroxybenzoate, NADP⁺, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is 3-hydroxybenzoate,NADPH:oxygen oxidoreductase (4-hydroxylating). This enzyme is also called 3-hydroxybenzoate 4-hydroxylase. This enzyme participates in benzoate degradation via hydroxylation and 2,4-dichlorobenzoate degradation. It employs one cofactor, FAD.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2DKH and 2DKI.