Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)

In enzymology, a bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) (EC 3.6.1.17) is an enzyme that catalyzes the chemical reaction

P₁,P₄-bis(5'-guanosyl) tetraphosphate + H₂O ${\displaystyle \rightleftharpoons }$ GTP + GMP

bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)
Identifiers
EC no.	3.6.1.17
CAS no.	37289-29-5
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Thus, the two substrates of this enzyme are P1,P4-bis(5'-guanosyl) tetraphosphate and H₂O, whereas its two products are GTP and GMP.

This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing anhydrides. The systematic name of this enzyme class is P1,P4-bis(5'-nucleosyl)-tetraphosphate nucleotidohydrolase. Other names in common use include bis(5'-guanosyl)-tetraphosphatase, bis(5'-adenosyl)-tetraphosphatase, diguanosinetetraphosphatase (asymmetrical), dinucleosidetetraphosphatase (asymmetrical), diadenosine P1,P4-tetraphosphatase, dinucleoside tetraphosphatase, and 1-P,4-P-bis(5'-nucleosyl)-tetraphosphate nucleotidohydrolase. This enzyme participates in purine metabolism and pyrimidine metabolism.

Structural studies

As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes 1F3Y, 1JKN, 1KT9, 1KTG, 1XSA, 1XSB, and 1XSC.