Undecaprenyl-diphosphatase

In enzymology, an undecaprenyl-diphosphatase (EC 3.6.1.27) is an enzyme that catalyzes the chemical reaction

undecaprenyl diphosphate + H₂O ${\displaystyle \rightleftharpoons }$ undecaprenyl phosphate + phosphate

undecaprenyl-diphosphatase
Identifiers
EC no.	3.6.1.27
CAS no.	9077-80-9
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Bacitracin resistance protein
Identifiers
Symbol	?
Pfam	PF02673
InterPro	IPR003824
OPM superfamily	479
OPM protein	5oon
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Thus, the two substrates of this enzyme are undecaprenyl diphosphate and H₂O, whereas its two products are undecaprenyl phosphate and phosphate. The enzymatic activity is enhanced by divalent cations, particularly Ca²⁺.

In many bacteria, this enzyme is a membrane protein that participates in peptidoglycan biosynthesis. The enzyme has been implicated in conferring resistance to the antibiotic bacitracin.^[1]

Nomenclature

This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing anhydrides. The systematic name of this enzyme class is undecaprenyl-diphosphate phosphohydrolase. Other names in common use include Undecaprenyl-pyrophosphate phosphatase (Uppp), UPP phosphatase, BacA, C55-isoprenyl diphosphatase, C55-isoprenyl pyrophosphatase, and isoprenyl pyrophosphatase.^[2]

Note: The enzyme Uppp/BacA (EC 3.6.1.27) has occasionally been incorrectly termed an "undecaprenol kinase".^[3] However, that name should be reserved for a distinct enzyme (EC 2.7.1.66), which catalyses the addition of a phosphate group from ATP to undecaprenol (C55-isoprenyl alcohol).

Structure

X-ray crystal structures of the membrane-form of the enzyme from E. coli^[4][5] are available (PDB IDs: 5OON,^[6] 6CB2^[7]).