Kelch motif

The Kelch motif is a region of protein sequence found widely in proteins from bacteria and eukaryotes.^[2] This sequence motif is composed of about 50 amino acid residues which form a structure of a four stranded beta-sheet "blade". This sequence motif is found in between five and eight tandem copies per protein which fold together to form a larger circular solenoid structure called a beta-propeller domain.

Kelch motif
Structure of Galactose oxidase containing kelch repeats.[1]
Identifiers
Symbol	Kelch_1
Pfam	PF01344
InterPro	IPR006652
SMART	Kelch
SCOP2	1gof / SCOPe / SUPFAM
OPM superfamily	319
OPM protein	3ii7
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Kelch motif
Identifiers
Symbol	Kelch_2
Pfam	PF07646
Pfam clan	CL0186
InterPro	IPR011498
SCOP2	1gof / SCOPe / SUPFAM
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Galactose oxidase, central domain
Identifiers
Symbol	Kelch_3
Pfam	PF13415
Pfam clan	CL0186
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Galactose oxidase, central domain
Identifiers
Symbol	Kelch_4
Pfam	PF13418
Pfam clan	CL0186
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Kelch motif
Identifiers
Symbol	Kelch_5
Pfam	PF13854
Pfam clan	CL0186
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Kelch motif
Identifiers
Symbol	Kelch_6
Pfam	PF13964
Pfam clan	CL0186
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Proteins containing Kelch motifs

See also: Human proteins containing Kelch motifs

The Kelch motif is widely found in eukaryotic and bacterial species. Notably the human genome contains around 100 proteins containing the Kelch motif. Within individual proteins the motif occurs multiple times. For example, the motif appears 6 times in Drosophila egg-chamber regulatory protein. The motif is also found in mouse protein MIPP^[3] and in a number of poxviruses. In addition, kelch repeats have been recognised in alpha- and beta-scruin,^[4][5] in galactose oxidase from the fungus Dactylium dendroides,^[6][7] and in the Escherichia coli NanM protein, a sialic acid mutarotase.^[8]

Structure

The structure of galactose oxidase reveals that the repeated Kelch sequence motif corresponds to a 4-stranded anti-parallel beta-sheet motif that forms the repeat unit in a super-barrel structural fold commonly known as a beta propeller.^[9]

Function

The known functions of kelch-containing proteins are diverse:

• scruin is an actin cross-linking protein;
• galactose oxidase catalyses the oxidation of the hydroxyl group at the C6 position in D-galactose;
• neuraminidase hydrolyses sialic acid residues from glycoproteins;
• NanM is a sialic acid mutarotase, involved in efficient utilisation of sialic acid by bacteria;
• kelch may have a cytoskeletal function, as it is localised to the actin-rich ring canals that connect the 15 nurse cells to the developing oocyte in Drosophila.^[4]

See also

• WD40 repeat
• Kelch protein