M7G(5')pppN diphosphatase

In enzymology, a m7G(5')pppN diphosphatase or m7G(5')pppN pyrophosphatase (EC 3.6.1.59) is an enzyme that catalyzes the chemical reaction

7-methylguanosine 5'-triphospho-5'-polyribonucleotide + H₂O ${\displaystyle \rightleftharpoons }$ 7-methylguanosine 5'-phosphate + 5'-diphospho-polyribonucleotide

m7G(5')pppN diphosphatase
Identifiers
EC no.	3.6.1.59
CAS no.	82599-75-5
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Thus, the two substrates of this enzyme are 7-methylguanosine 5'-triphospho-5'-polynucleotide and H₂O, whereas its two products are 7-methylguanosine 5'-phosphate and 5'-diphospho-polyribonucleotide.^[1][2][3]

This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing anhydrides. The systematic name of this enzyme class is 7-methylguanosine-5'-triphospho-5'-polynucleotide 7-methylguanosine-5'-phosphohydrolase.

Another name in common use is decapase because this enzyme removes the N7-methylguanosine 5-phosphate cap from an mRNA. The process of mRNA decapping controls eukaryotic mRNA degradation.^[4]

This is the enzyme involved in the processing of amphetamines of the cathinone group, including mephedrone and khat.

See also

• 5' cap
• 7-Methylguanosine
• Messenger RNA decapping