2-Hydroxy-3-morpholinopropanesulfonic acid

MOPSO is a zwitterionic organic chemical buffering agent; one of Good's buffers. MOPSO and MOPS (3-morpholinopropanesulfonic acid) are chemically similar, differing only in the presence of a hydroxyl group on the C-2 of the propane moiety. It has a useful pH range of 6.5-7.9 in the physiological range, making it useful for cell culture work. It has a pKa of 6.9 with ΔpKa/°C of -0.015 and a solubility in water at 0°C of 0.75 M.

MOPSO

Chemical structure of MOPSO
Names
Preferred IUPAC name 2-Hydroxy-3-(morpholin-4-yl)propane-1-sulfonic acid[1][2]
Other names MOPSO, 3-morpholino-2-hydroxypropanesulfonic acid, 3-morpholino-2HOPSA
Identifiers
CAS Number	68399-77-9 Y
3D model (JSmol)	Interactive image
ChEBI	CHEBI:190979
ChemSpider	98300
ECHA InfoCard	100.063.607
EC Number	269-989-6
PubChem CID	109333
UNII	6XB7PS9THL Y
CompTox Dashboard (EPA)	DTXSID40887352
InChI InChI=1S/C7H15NO5S/c9-7(6-14(10,11)12)5-8-1-3-13-4-2-8/h7,9H,1-6H2,(H,10,11,12) Key: NUFBIAUZAMHTSP-UHFFFAOYSA-N
SMILES C1COCCN1CC(CS(=O)(=O)O)O
Properties
Chemical formula	C7H15NO5S
Molar mass	225.259 g/mol
Appearance	White crystalline powder
Melting point	270–290 °C (518–554 °F; 543–563 K)
Acidity (pKa)	6.9[3]
Hazards
Occupational safety and health (OHS/OSH):
Main hazards	Irritant
GHS labelling:
Pictograms
Signal word	Warning
Hazard statements	H315, H319, H335
Precautionary statements	P261, P264, P271, P280, P302+P352, P304+P340, P305+P351+P338, P312, P321, P332+P313, P337+P313, P362, P403+P233, P405, P501
Flash point	Non-flammable
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). Infobox references

MOPSO has been used as a buffer component for:^[4]

• Copper analysis via:
  • Flow injection micellar technique of the catalytic reaction of 3-methyl-2-benzothiazolinone hydrazone and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline
  • Electrospray ionization quadrupole time-of-flight mass spectroscopy to measure MOPSO-copper chelated complexes
• Discontinuous gel electrophoresis on rehydratable polyacrylamide gels
• Buffered charcoal yeast extract agar
• Fixing cells in urine in a buffered alcohol
• Testing crude oil bioremediation products in marine environments

See also

• MOPS
• MES
• CAPS
• CHES