NAD+ diphosphatase

In enzymology, a NAD⁺ diphosphatase (EC 3.6.1.22) is an enzyme that catalyzes the chemical reaction

NAD⁺ + H₂O ${\displaystyle \rightleftharpoons }$ AMP + NMN

NAD+ diphosphatase
Identifiers
EC no.	3.6.1.22
CAS no.	37289-33-1
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Thus, the two substrates of this enzyme are NAD⁺ and H₂O, whereas its two products are AMP and NMN.

This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing anhydrides. The systematic name of this enzyme class is NAD⁺ phosphohydrolase. Other names in common use include nicotinamide adenine dinucleotide pyrophosphatase, NADP+ pyrophosphatase, and NADH pyrophosphatase. This enzyme participates in nicotinate and nicotinamide metabolism.

Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 2GB5.