NAD+ synthase

In enzymology, a NAD⁺ synthetase (EC 6.3.1.5) is an enzyme that catalyzes the chemical reaction

ATP + deamido-NAD⁺ + NH₃ ${\displaystyle \rightleftharpoons }$ AMP + diphosphate + NAD⁺

NAD+ synthetase
Identifiers
EC no.	6.3.1.5
CAS no.	9032-69-3
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

The 3 substrates of this enzyme are ATP, deamido-NAD⁺, and NH₃, whereas its 3 products are AMP, diphosphate, and NAD⁺.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-ammonia (or amine) ligases (amide synthetase). The systematic name of this enzyme class is deamido-NAD⁺:ammonia ligase (AMP-forming). Other names in common use include NAD⁺ synthetase, NAD⁺ synthetase, nicotinamide adenine dinucleotide synthetase, and diphosphopyridine nucleotide synthetase. This enzyme participates in nicotinate and nicotinamide metabolism and nitrogen metabolism.

Structural studies

As of late 2007, 11 structures have been solved for this class of enzymes, with PDB accession codes 1WXE, 1WXF, 1WXG, 1WXH, 1WXI, 1XNG, 1XNH, 2E18, 2PZ8, 2PZA, and 2PZB.