Pyrrolysine—tRNAPyl ligase

Pyrrolysine—tRNA^Pyl ligase (EC 6.1.1.26, PylS, pyrrolysyl-tRNA synthetase) is an enzyme with systematic name L-pyrrolysine:tRNAPyl ligase (AMP-forming).^[1][2][3] This enzyme catalyses the following chemical reaction

ATP + L-pyrrolysine + tRNAPyl ${\displaystyle \rightleftharpoons }$ AMP + diphosphate + L-pyrrolysyl-tRNAPyl

Pyrrolysine—tRNAPyl ligase
Identifiers
EC no.	6.1.1.26
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

This enzyme is specific for pyrrolysine as substrate as it cannot be replaced by lysine or any of the other natural amino acids.