TRNA (guanine-N1-)-methyltransferase

In enzymology, a tRNA (guanine-N1-)-methyltransferase (EC 2.1.1.31) is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + tRNA ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + tRNA containing N₁-methylguanine

tRNA (guanine-N1-)-methyltransferase
Identifiers
EC no.	2.1.1.31
CAS no.	37256-99-8
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA, whereas its two products are S-adenosylhomocysteine and tRNA containing N1-methylguanine.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA (guanine-N1-)-methyltransferase. Other names in common use include transfer ribonucleate guanine 1-methyltransferase, tRNA guanine 1-methyltransferase, and S-adenosyl-L-methionine:tRNA (guanine-1-N-)-methyltransferase.

Structural studies

As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes 1OY5, 1P9P, 1UAJ, 1UAK, 1UAL, and 1UAM.