Turk's solution

In hemocytometry, Türk's solution (or Türk's fluid) is a hematological stain (either crystal violet or aqueous methylene blue) prepared in 99% acetic acid (glacial)^[1] and distilled water. The solution destroys the red blood cells and platelets within a blood sample (acetic acid being the main lyzing agent), and stains the nuclei of the white blood cells, making them easier to see and count.^[1]

Türk's solution in a laboratory bottle

Türk's solution is intended for use in determining total leukocyte count in a defined volume of blood. Erythrocytes are hemolyzed while leukocytes are stained for easy visualization.^[1][2]

Composition of Türk's solution is as follows:

Component	Volume
Crystal violet	1 mL[1]
Glacial acetic acid	1.5 mL[1]
Distilled water	100 mL[1]