Y-27632

Y-27632 is a biochemical tool used in the study of the rho-associated protein kinase (ROCK) signaling pathways.^[1] Y-27632 selectively inhibits p160ROCK, although it does inhibit other protein kinases such as PKCs at higher concentrations.^[2]

Y-27632

Names
IUPAC name (1R,4r)-4-((R)-1-Aminoethyl)-N-(pyridin-4-yl)cyclohexanecarboxamide
Identifiers
CAS Number	146986-50-7 Y
3D model (JSmol)	Interactive image
ChEBI	CHEBI:75393 N
ChEMBL	ChEMBL2218937 N
ChemSpider	20016532 Y
DrugBank	DB08756 Y
ECHA InfoCard	100.149.247
IUPHAR/BPS	5290
PubChem CID	448042
UNII	0X370ROP6H Y
CompTox Dashboard (EPA)	DTXSID7043740
InChI InChI=1S/C14H21N3O/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13/h6-12H,2-5,15H2,1H3,(H,16,17,18)/t10-,11-,12-/m1/s1 Y Key: IYOZTVGMEWJPKR-IJLUTSLNSA-N Y InChI=1/C14H21N3O/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13/h6-12H,2-5,15H2,1H3,(H,16,17,18)/t10-,11-,12-/m1/s1 Key: IYOZTVGMEWJPKR-IJLUTSLNBP
SMILES O=C([C@H]1CC[C@@]([C@H](N)C)([H])CC1)NC2=CC=NC=C2
Properties
Chemical formula	C14H21N3O
Molar mass	247.342 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). N verify (what is YN ?) Infobox references

It has been studied for its effects on corneal endothelial cells (CECs) ^[3] and cardiac stem cells (CSCs).^[4]

The substance has been used as part of a chemical cocktail to turn old and senescent human cells back into young ones (as measured by transcriptomic age), without turning them all the way back into undifferentiated stem cells.^[5]

A mixture of methylcellulose (1%), ethylene glycol (10%), dimethyl sulfoxide (10%), and Y-27632 (10 μM), termed MEDY, has been shown to be an effective cryopreservation reagent for brain tissue, enabling tissue to resume growth and function after freezing using liquid nitrogen.^[6]