dnaC
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dnaC is a loading factor that complexes with the C-terminus of helicase dnaB and inhibits it from unwinding the dsDNA at a replication fork.[1] A dnaB and dnaC associate near the dnaA bound origin for each of the ssDNA.[1] One dnaB-dnaC complex is oriented in the opposite direction to the other dnaB-dnaC complex due to the antiparallel nature of DNA. Because they are oriented in opposite directions, one dnaB-dnaC complex will complex with dnaA from the N-terminus of dnaB whereas the other dnaB-dnaC complex will complex with dnaA from the dnaC. After the assembly of dnaG onto the N-terminus of dnaB, dnaC is released and dnaB will be allowed to begin unwinding dsDNA to make room for DNA polymerase III to begin synthesizing the daughter strands.[1]
DNA replication gene dnaC | |||||||
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Identifiers | |||||||
Organism | Escherichia coli (str. K-12 substr. MG1655) | ||||||
Symbol | dnaC | ||||||
Entrez | 948864 | ||||||
RefSeq (Prot) | NP_418781.1 | ||||||
UniProt | P0AEF0 | ||||||
Other data | |||||||
EC number | 2.7.7.7 | ||||||
Chromosome | genome: 4.6 - 4.6 Mb | ||||||
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This interaction of dnaC with dnaB requires the hydrolysis of ATP.[2]