Ames Main Street Historic District is a nationally recognized historic district located in Ames, Iowa, United States. It was listed on the National Register of Historic Places in 2018. At the time of its nomination it included 81 resources as well as 15 non-contributing buildings. The district covers most of the city's central business district. The area began to develop after the Civil War along the Chicago and North Western (CNW) tracks. The district was also served by the Ames & College Railway and its successor, the Fort Dodge, Des Moines, & Southern Interurban Streetcar line, from 1891 to 1929. The later connected downtown Ames and the CNW depot with Iowa State College, now known as Iowa State University.