Brothertown is a town in Calumet County in the U.S. state of Wisconsin. The population was 1,328 at the 2020 census, slightly down from 1,329 at the 2010 census. The unincorporated communities of Brothertown, Charlesburg, Eckers Lakeland, Jericho, and Maple Heights are located in the town. The unincorporated community of Calumetville is also located partially in the town.