Melbourne tram route 16 is a tram route on the Melbourne tramway network serving the city of Melbourne in Victoria, Australia. Operated by Yarra Trams, the route is coloured light yellow and extends from Melbourne University to Kew over 20.2 kilometre of double track via Swanston Street, St Kilda Road, Fitzroy Street, The Esplanade, Balaclava Road and Glenferrie Road. It is serviced out of Malvern depot utilising Z and D1 class trams.