Presquile National Wildlife Refuge in the U.S. state of Virginia is one of four refuges that make up the Eastern Virginia Rivers National Wildlife Refuge Complex. It is managed by the United States Fish and Wildlife Service. The refuge is a 1,329-acre (5.38 km2) island in the James River, located approximately 20 miles (32 km) south of Richmond. It is located in the easternmost part of Chesterfield County, northeast of Hopewell.