The St. Croix Subdivision or St. Croix Sub is an American rail line operated by the BNSF Railway. It runs approximately 150 miles (240 km) from La Crosse, Wisconsin to Saint Paul, Minnesota, following the Mississippi River. It runs through the communities of La Crosse, Onalaska, Brice Prairie, Trempealeau, East Winona, Fountain City, Cochrane, Buffalo City, Alma, Nelson, Pepin, Stockholm, Maiden Rock, Bay City, Diamond Bluff, and Prescott, Wisconsin. This section of track sees 55-60 trains a day and is double tracked for most of the 150 miles with exceptions of Burns to Prescott, Mears to Trevino, and Winona Jct. to East Winona. The BNSF vertical lift bridge over the mouth of the St. Croix river is single-tracked.