4-ヒドロキシ安息香酸-3-モノオキシゲナーゼ

4-ヒドロキシ安息香酸-3-モノオキシゲナーゼ（4-hydroxybenzoate 3-monooxygenase）は、次の化学反応を触媒する酸化還元酵素である。

4-ヒドロキシ安息香酸 + NADPH + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ プロトカテク酸 + NADP⁺ + H₂O

4-ヒドロキシ安息香酸-3-モノオキシゲナーゼ
識別子
EC番号	1.14.13.2
CAS登録番号	9059-23-8
データベース
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB構造	RCSB PDB PDBj PDBe PDBsum
遺伝子オントロジー	AmiGO / QuickGO
検索 PMC articles PubMed articles NCBI proteins

反応式の通り、この酵素の基質は4-ヒドロキシ安息香酸とNADPHとH⁺とO₂、生成物はプロトカテク酸とNADP⁺とH₂Oである。補酵素としてFADを用いる。

組織名は4-hydroxybenzoate,NADPH:oxygen oxidoreductase (3-hydroxylating)で、別名にp-hydroxybenzoate hydrolyase、p-hydroxybenzoate hydroxylase、4-hydroxybenzoate 3-hydroxylase、4-hydroxybenzoate monooxygenase、4-hydroxybenzoic hydroxylase、p-hydroxybenzoate-3-hydroxylase、p-hydroxybenzoic acid hydrolase、p-hydroxybenzoic acid hydroxylase、p-hydroxybenzoic hydroxylaseがある。

参考文献

• Hosokawa K, Stanier RY (1966). “Crystallization and properties of p-hydroxybenzoate hydroxylase from Pseudomonas putida”. J. Biol. Chem. 241 (10): 2453–60. PMID 4380381.
• Howell LG, Spector T, Massey V (1972). “Purification and properties of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens”. J. Biol. Chem. 247 (13): 4340–50. PMID 4402514.
• Spector T, Massey V (1972). “Studies on the effector specificity of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens”. J. Biol. Chem. 247 (14): 4679–87. PMID 4402938.
• Spector T, Massey V (1972). “p-Hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Evidence for an oxygenated flavin intermediate”. J. Biol. Chem. 247 (17): 5632–6. PMID 4403446.
• Spector T, Massey V (1972). “p-Hydroxybenzoate hydroxylase from Pseudomonas fluorescens Reactivity with oxygen”. J. Biol. Chem. 247 (22): 7123–7. PMID 4404745.
• Seibold B, Matthes M, Eppink MH, Lingens F, Van Berkel WJ, Muller R (1996). “4-Hydroxybenzoate hydroxylase from Pseudomonas sp. CBS3 Purification, characterization, gene cloning, sequence analysis and assignment of structural features determining the coenzyme specificity”. Eur. J. Biochem. 239 (2): 469–78. doi:10.1111/j.1432-1033.1996.0469u.x. PMID 8706756.