| Placering | Heat | Bana | Nation | Simmare | Tid | Noteringar |
| 1 | 1 | 4 |  USA | Hunter Armstrong (48,37)
Jack Aikins (48,42)
Luke Hobson (47,70)
Carson Foster (47,83) | 3.12,32 | 0Q0 |
| 2 | 2 | 4 |  Italien | Lorenzo Zazzeri (48,33)
Paolo Conte Bonin (48,16)
Leonardo Deplano (48,59)
Alessandro Miressi (47,88) | 3.12,96 | 0Q0 |
| 3 | 2 | 0 |  Storbritannien | Jacob Whittle (48,82)
Tom Dean (49,13)
Duncan Scott (48,25)
Matthew Richards (47,76) | 3.13,96 | 0Q0 |
| 4 | 2 | 5 |  Kina | Wang Haoyu (48,08)
Ji Xinjie (48,10)
Zhang Zhanshuo (49,63)
Pan Zhanle (48,26) | 3.14,07 | 0Q0 |
| 5 | 2 | 7 |  Grekland | Apostolos Christou (48,92)
Stergios Bilas (48,81)
Kristian Golomeev (47,86)
Andreas Vazaios (48,74) | 3.14,33 | 0Q0 |
| 6 | 2 | 6 |  Serbien | Velimir Stjepanović (48,87)
Andrej Barna (47,63)
Uroš Nikolić (49,60)
Nikola Aćin (48,32) | 3.14,42 | 0Q0 |
| 7 | 1 | 3 |  Spanien | Luis Domínguez (49,02)
Sergio de Celis (48,11)
Mario Mollà (48,28)
César Castro (49,30) | 3.14,71 | 0Q0 |
| 8 | 1 | 6 |  Ungern | Szebasztián Szabó (49,32)
Dániel Mészáros (48,69)
Adam Jaszo (48,43)
Nandor Nemeth (48,29) | 3.14,73 | 0Q0 |
| 9 | 1 | 7 |  Sverige | Robin Hanson (49,27)
Björn Seeliger (47,97)
Isak Eliasson (49,09)
Marcus Holmquist (49,04) | 3.15,37 | |
| 10 | 2 | 2 |  Polen | Kamil Sieradzki (48,91)
Ksawery Masiuk (48,47)
Mateusz Chowaniec (48,76)
Kacper Majchrzak (49,57) | 3.15,71 | |
| 11 | 1 | 5 |  Kanada | Javier Acevedo (48,99)
Finlay Knox (47,96)
Antoine Sauvé (49,38)
Stephen Calkins (49,41) | 3.15,74 | |
| 12 | 2 | 3 |  Sydkorea | Yang Jae-hoon (49,28)
Ji Yu-chan (48,90)
Lee Yoo-yeon (48,69)
Kim Min-suk (50,24) | 3.17,11 | |
| 13 | 1 | 1 |  Litauen | Tomas Navikonis (49,00)
Daniil Pancerevas (49,91)
Tomas Lukminas (49,10)
Rokas Jazdauskas (49,40) | 3.17,41 | |
| 14 | 1 | 2 |  Singapore | Mikkel Lee (49,64)
Jonathan Tan (48,87)
Ardi Azman (49,52)
Darren Lim (50,49) | 3.18,52 | |
| 15 | 2 | 1 |  Mexiko | Andres Dupont (48,81)
Héctor Ruvalcaba (50,62)
José Ángel Martínez (50,65)
Jorge Iga (50,33) | 3.20,41 | |
| 16 | 1 | 0 |  Bulgarien | Josif Miladinov (50,43)
Kaloyan Bratanov (49,42)
Petar Mitsin (51,08)
Yordan Yanchev (49,60) | 3.20,53 | 0NR0 |
| 17 | 2 | 9 |  Slovakien | Tibor Tistan (50,82)
Matej Duša (50,35)
Richard Nagy (52,29)
Frantisek Jablcnik (51,24) | 3.24,70 | 0NR0 |
| 18 | 1 | 8 |  Thailand | Dulyawat Kaewsriyong (50,88)
Navaphat Wongcharoen (52,04)
Ratthawit Thammananthachote (52,29)
Tonnam Kanteemool (51,38) | 3.26,59 | |
| 19 | 2 | 8 |  Vietnam | Trần Hưng Nguyên (51,32)
Nguyễn Huy Hoàng (54,04)
Ngô Đình Chuyền (51,98)
Nguyễn Quang Thuấn (53,03) | 3.30,37 | |
