猪笼草蛋白酶

猪笼草蛋白酶（Nepenthesin，Nepenthacin^[1][2]，Nepenthasin^[3]），又称猪笼草酸性蛋白酶，猪笼草天冬氨酸蛋白酶^[3]是猪笼草捕虫笼及盾叶茅膏菜（Drosera peltata）分泌的天冬氨酸蛋白酶。^{[4][5][6][7][8][9]}其与胃蛋白酶类似，但其也能酶切两侧的天冬氨酸残基及赖氨酸┼精氨酸。^[3]其对pH值及温度的稳定性高于猪胃蛋白酶A，对尿素及盐酸胍的稳定性大大降低。^[10]

猪笼草蛋白酶
命名 系统命名 缩写
识别码
EC编号	3.4.23.12
CAS号	9073-80-7
数据库
IntEnz	IntEnz浏览
BRENDA（英语：BRENDA）	BRENDA入口
ExPASy（英语：ExPASy）	NiceZyme浏览
KEGG	KEGG入口
MetaCyc（英语：MetaCyc）	代谢路径
PRIAM（英语：PRIAM_enzyme-specific_profiles）	概述
PDB	RCSB PDB PDBj PDBe PDBsum
搜索 PMC 相关文献 PubMed 相关文献

1968年，Shigeru Nakayama和Shizuko Amagase以“nepenthesin”命名猪笼草蛋白酶。^[11]在猪笼草属中已确定了2个同工酶：猪笼草蛋白酶I与猪笼草蛋白酶II。^[12]

参考文献

1. Jentsch J. Enzymes from carnivorous plants (nepenthes). Isolation of the protease nepenthacin. FEBS Lett. April 1972, 21 (3): 273–276. PMID 11946525. doi:10.1016/0014-5793(72)80181-9.
2. Jentsch J, Meierkord S, Hammer M. The enzymes from carnivorous plants (Nepenthes): Properties and characterization of the acid protease nepenthacin. Planta Medica. 1989, 55: 227. doi:10.1055/s-2006-961979.
3. EC 3.4.23.12 - Nepenthesin （页面存档备份，存于互联网档案馆）. Integrated Enzyme Database (IntEnz).
4. Amagase S, Nakayama S, Tsugita A. Acid protease in Nepenthes. II. Study on the specificity of nepenthesin. J. Biochem. October 1969, 66 (4): 431–9. PMID 5354017.
5. Amagase S. Digestive enzymes in insectivorous plants. 3. Acid proteases in the genus Nepenthes and Drosera peltata. J. Biochem. July 1972, 72 (1): 73–81. PMID 5069751.
6. Amagase S, Mori M, Nakayama S. Digestive enzymes in insectivorous plants. IV. Enzymatic digestion of insects by Nepenthes secretion and Drosera peltata extract: proteolytic and chitinolytic activities. J. Biochem. September 1972, 72 (3): 765–7. PMID 4634982.
7. Tökés ZA, Woon WC, Chambers SM. Digestive enzymes secreted by the carnivorous plant Nepenthes macferlanei L. Planta. March 1974, 119 (1): 39–46. doi:10.1007/BF00390820.
8. Athauda SBP, Inoue H, Iwamatsu A, Takahashi K. Acid Proteinase from Nepenthes distillatoria (Badura). James, Michael (编). Aspartic proteinases: retroviral and cellular enzymes. New York: Plenum. 1998: 453–458. ISBN 0-306-45809-8.
9. Takahashi K, Tanji M, Shibata C. Variations in the content and isozymic composition of nepenthesin in the pitcher fluids among Nepenthes species (PDF). Carnivorous Plant News Letter. 2007, 36 (3): 73–76 [2011-12-10]. （原始内容存档 (PDF)于2012-02-27）.
10. Kubota K, Metoki Y, Athauda SBP, Shibata C, Takahashi K. Stability Profiles of Nepenthesin in Urea and Guanidine Hydrochloride: Comparison with Porcine Pepsin A. Bioscience, Biotechnology, and Biochemistry. 2010, 74 (11): 2323–2326. doi:10.1271/bbb.100391.
11. Nakayama S, Amagase S. Acid Protease in Nepenthes: Partial Purification and Properties of the Enzyme. Proceedings of the Japan Academy. 1968, 44 (5): 358–362.^{[永久失效链接]}
12. Athauda SB, Matsumoto K, Rajapakshe S, Kuribayashi M, Kojima M, Kubomura-Yoshida N, Iwamatsu A, Shibata C, Inoue H, Takahashi K. Enzymic and structural characterization of nepenthesin, a unique member of a novel subfamily of aspartic proteinases. Biochem. J. July 2004, 381 (Pt 1): 295–306. PMC 1133788 . PMID 15035659. doi:10.1042/BJ20031575.

扩展阅读

• The MEROPS online database for peptidases and their inhibitors: A01.040