Beta-pyrazolylalanine synthase

In enzymology, a beta-pyrazolylalanine synthase (EC 2.5.1.51) is an enzyme that catalyzes the chemical reaction

O₃-acetyl-L-serine + pyrazole ${\displaystyle \rightleftharpoons }$ 3-(pyrazol-1-yl)-L-alanine + acetate

beta-pyrazolylalanine synthase
Identifiers
EC no.	2.5.1.51
CAS no.	37290-81-6
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Thus, the two substrates of this enzyme are O3-acetyl-L-serine and pyrazole, whereas its two products are 3-(pyrazol-1-yl)-L-alanine and acetate.

This enzyme belongs to the family of transferases, specifically those transferring aryl or alkyl groups other than methyl groups. The systematic name of this enzyme class is O3-acetyl-L-serine:pyrazole 1-(2-amino-2-carboxyethyl)transferase. Other names in common use include beta-(1-pyrazolyl)alanine synthase, beta-pyrazolealanine synthase, beta-pyrazolylalanine synthase (acetylserine), O3-acetyl-L-serine acetate-lyase (adding pyrazole), BPA-synthase, pyrazolealanine synthase, pyrazolylalaninase, and 3-O-acetyl-L-serine:pyrazole 1-(2-amino-2-carboxyethyl)transferase.

References

• Murakoshi I, Ikegami F, Hinuma Y, Hanma Y (1984). "Purification and characterization of beta-(pyrazol-1-yl)-L-alanine synthase from Citrullus vulgaris". Phytochemistry. 23 (5): 973–977. doi:10.1016/S0031-9422(00)82594-7.
• Murakoshi I, Ikegami F, Hinuma Y, Hanma Y (1984). "Purification and characterization of L-mimosine synthase from Leucaena leucocephala". Phytochemistry. 23 (9): 1905–1908. doi:10.1016/S0031-9422(00)84938-9.
• Murakoshi I, Kuramoto H, Haginiwa J (1972). "The enzymic synthesis of beta-substituted alanines". Phytochemistry. 11: 177–182. doi:10.1016/S0031-9422(00)89986-0.
• Noji M, Murakoshi I, Saito K (1993). "Evidence for identity of beta-pyrazolealanine synthase with cysteine synthase in watermelon: formation of beta-pyrazole-alanine by cloned cysteine synthase in vitro and in vivo". Biochem. Biophys. Res. Commun. 197 (3): 1111–7. doi:10.1006/bbrc.1993.2592. PMID 8280125.