3alpha-hydroxysteroid dehydrogenase (B-specific)

In enzymology, a 3alpha-hydroxysteroid dehydrogenase (B-specific) (EC 1.1.1.50) is an enzyme that catalyzes the chemical reaction

Androsterone

+ NAD⁺

 

 

 

H⁺

 

H⁺

 

5α-Androstane-3,17-dione

+ NADH

 

3-alpha-hydroxysteroid dehydrogenase (B-specific)
Identifiers
EC no.	1.1.1.50
CAS no.	9028-56-2
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

The two substrates of this enzyme are androsterone and oxidised nicotinamide adenine dinucleotide (NAD⁺). Its products are 5α-androstane-3,17-dione, reduced NADH, and a proton. The alternative cofactor nicotinamide adenine dinucleotide phosphate is also used by this enzyme.^{[1][2][3][4][5]}

The enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor, more specifically it is part of the group of hydroxysteroid dehydrogenases. The systematic name of this enzyme class is 3alpha-hydroxysteroid:NAD(P)⁺ oxidoreductase (B-specific). Other names in common use include hydroxyprostaglandin dehydrogenase, 3alpha-hydroxysteroid oxidoreductase, and sterognost 3alpha. This enzyme participates in 3 metabolic pathways: bile acid biosynthesis, c21-steroid hormone metabolism, and androgen and estrogen metabolism.

Structural studies

As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes 1AFS, 1FJH, 1FK8, 1LWI, 1RAL, 2DKN, and 2FVL.