4-Hydroxy-3-methylbut-2-enyl diphosphate reductase

4-Hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2, isopentenyl-diphosphate:NADP+ oxidoreductase, LytB, (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase, HMBPP reductase, IspH, LytB/IspH) is an enzyme in the non-mevalonate pathway. It acts upon (E)-4-Hydroxy-3-methyl-but-2-enyl pyrophosphate (or "HMB-PP").^{[1][2][3][4][5][6]}

(1) isopentenyl diphosphate + NAD(P)⁺ + H₂O ${\displaystyle \rightleftharpoons }$ (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H⁺

(2) dimethylallyl diphosphate + NAD(P)⁺ + H₂O ${\displaystyle \rightleftharpoons }$ (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H⁺

4-Hydroxy-3-methylbut-2-enyl diphosphate reductase
Identifiers
EC no.	1.17.1.2
CAS no.	512789-14-9
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

4-Hydroxy-3-methylbut-2-enyl diphosphate reductase is an iron-sulfur protein that contains either a [3Fe-4S] or a [4Fe-4S] cluster.