ANTH domain

The ANTH domain is a membrane binding domain that shows weak specificity for PtdIns(4,5)P2. It was found in AP180 (homologous to CALM^[1]) endocytotic accessory protein that has been implicated in the formation of clathrin-coated pits. The domain is involved in phosphatidylinositol 4,5-bisphosphate binding and is a universal adaptor for nucleation of clathrin coats.^[2][3]

ANTH domain
Clathrin assembly lymphoid myeloid leukemia (CALM) protein
Identifiers
Symbol	ANTH
Pfam	PF07651
InterPro	IPR011417
OPM superfamily	38
OPM protein	1hfa
CDD	cd03564
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Its structure is a solenoid of 9 helices. The PtdIns(4,5)P2 binding residues are spread over several helices at the tip of the structure. The PtdIns(4,5)P2 binding sequence is Kx9Kx(K/R)(H/Y).

An ANTH domain is also found in HIP1 and HIP1R, and the PtdIns(4,5)P2 binding sequence is conserved.

Human proteins containing this domain

HIP1; HIP1R; PICALM; SNAP91;