Asparagine synthase (glutamine-hydrolysing)

Asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4, asparagine synthetase (glutamine-hydrolysing), glutamine-dependent asparagine synthetase, asparagine synthetase B, AS, AS-B) is an enzyme with systematic name L-aspartate:L-glutamine amido-ligase (AMP-forming).^{[1][2][3][4][5][6]} This enzyme catalyses the following chemical reaction

ATP + L-aspartate + L-glutamine + H₂O ${\displaystyle \rightleftharpoons }$ AMP + diphosphate + L-asparagine + L-glutamate (overall reaction)

(1a) L-glutamine + H₂O ${\displaystyle \rightleftharpoons }$ L-glutamate + NH₃

(1b) ATP + L-aspartate + NH₃ ${\displaystyle \rightleftharpoons }$ AMP + diphosphate + L-asparagine

Asparagine synthase (glutamine-hydrolysing)
Asparagine synthetase B dimer, E.Coli
Identifiers
EC no.	6.3.5.4
CAS no.	37318-72-2
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

The enzyme from Escherichia coli has two active sites.^[6]