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Chu 13
Culture medium used in microbiology From Wikipedia, the free encyclopedia
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CHU 13 medium is a culture medium used in microbiology for the growth of certain algal species, first published by S.P. Chu in 1942.[1] It is used as growth medium for the biofuel candidate alga Botryococcus braunii.[2]

CHU 13 includes essential minerals and trace elements that are required by algae for growth, but does not include a carbon source and so is only appropriate for growth of phototrophs. It can be prepared as either a liquid medium or as an agar medium.
The remaining volume is pure, de-ionized water.
Because it is difficult to weigh out some of the trace minerals, it is advisable to create a mixture of all components at a large concentration, such as a thousand times these measures, and then mix with the appropriate amount of (pure, de-ionized) water. Correct pH to 7.5, and then autoclave.
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