DNA damage-binding protein

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DNA damage-binding protein or UV-DDB[1] is a protein complex that is responsible for repair of UV-damaged DNA.[2] This complex is composed of two protein subunits, a large subunit DDB1 (p127) and a small subunit DDB2 (p48). When cells are exposed to UV radiation, DDB1 moves from the cytosol to the nucleus and binds to DDB2, thus forming the UV-DDB complex. This complex formation is highly favorable and it is demonstrated by UV-DDB's binding preference and high affinity to the UV lesions in the DNA.[3] This complex functions in nucleotide excision repair, recognising UV-induced (6-4) pyrimidine-pyrimidone photoproducts and cyclobutane pyrimidine dimers.[1]

Quick Facts damage-specific DNA binding protein 1, 127kDa, Identifiers ...
damage-specific DNA binding protein 1, 127kDa
Identifiers
SymbolDDB1
Alt. symbolsXPE
NCBI gene1642
HGNC2717
OMIM600045
RefSeqNM_001923
UniProtQ16531
Other data
LocusChr. 11 q12-q13
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StructuresSwiss-model
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Quick Facts damage-specific DNA binding protein 2, 48kDa, Identifiers ...
damage-specific DNA binding protein 2, 48kDa
Identifiers
SymbolDDB2
Alt. symbolsDBB, UV-DDB2, FLJ34321
NCBI gene1643
HGNC2718
OMIM600811
RefSeqNM_000107
UniProtQ92466
Other data
LocusChr. 11 p12-p11
Search for
StructuresSwiss-model
DomainsInterPro
Close

Structure

The helical domain at the n-terminus of DDB2 binds to UV damaged DNA with high affinity to form the UV-DDB complex. The helical binding interaction at the n-terminus of DDB2 allows for the protein to bind immediately after detecting UV damaged DNA.[3] DNA binds to DDB2 only when damaged by UV radiation. Binding with high affinity to a helical domain of DDB2 in the dimer form, UV-DDB, is facilitated by the n-terminal alpha helical paddle and beta wings of the DDB2 subunit.[3] Both the alpha helical fold and the beta wing loops form a "winged helix" motif.[3] The dimerized complex acts as a scaffold for DNA damage repair pathways and allows for other proteins to detect, interact, and repair UV damaged DNA.

DDB2

DDB2 is a protein part of the CUL4A–RING ubiquitin ligase (CRL4) complex. It was thought that DDB2 only acts to recognize legions of UV damaged DNA. It has been found that DDB2 plays a role in promoting chromatin unfolding.[4] This role is independent of DDB2's role in the CRL4 complex.

Damage sensor role

UV-DDB is not only responsible for the repair of damaged DNA, it can also function by acting as a damage sensor.[5] In base excision repair, UV-DDB galvanizes OGG1 and APE 1 activities.[5] During DNA damage, proteins OGG1 and APE 1 encounter difficulty in repairing the lesions in a DNA wrapped nucleosome. Additionally, histones function by making the DNA inaccessible because of the way they make DNA coil and wrap into chromatin. UV-DDP plays a role in identifying the damaged sites within the chromatin, thereby allowing access to base excision repair proteins. When UV-DDB is recruited to these damaged sites, it recognizes the OGG1- AP DNA complex and further accelerates the turnover of glycosylases.[5]

References

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