Deoxyhypusine synthase

Deoxyhypusine synthase (EC 2.5.1.46, spermidine:eIF5A-lysine 4-aminobutyltransferase (propane-1,3-diamine-forming)) is an enzyme with systematic name (eIF5A-precursor)-lysine:spermidine 4-aminobutyltransferase (propane-1,3-diamine-forming).^{[1][2][3][4][5][6][7][8][9]} This enzyme catalyses the following chemical reaction

[eIF5A-precursor]-lysine + spermidine ${\displaystyle \rightleftharpoons }$ [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine (overall reaction)

(1a) spermidine + NAD⁺ ${\displaystyle \rightleftharpoons }$ dehydrospermidine + NADH

(1b) dehydrospermidine + [enzyme]-lysine ${\displaystyle \rightleftharpoons }$ N-(4-aminobutylidene)-[enzyme]-lysine + propane-1,3-diamine

(1c) N-(4-aminobutylidene)-[enzyme]-lysine + [eIF5A-precursor]-lysine ${\displaystyle \rightleftharpoons }$ N-(4-aminobutylidene)-[eIF5A-precursor]-lysine + [enzyme]-lysine

(1d) N-(4-aminobutylidene)-[eIF5A-precursor]-lysine + NADH + H+ ${\displaystyle \rightleftharpoons }$ [eIF5A-precursor]-deoxyhypusine + NAD⁺

Deoxyhypusine synthase
Identifiers
EC no.	2.5.1.46
CAS no.	127069-31-2
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

The eukaryotic initiation factor eIF5A contains a hypusine residue that is essential for activity.