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Eosin methylene blue

Culture medium in microbiology From Wikipedia, the free encyclopedia

Eosin methylene blue
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Eosin methylene blue (EMB, also known as "Levine's formulation") is a selective and differential media used for the identification of Gram-negative bacteria,[1] specifically the Enterobacteriaceae. EMB inhibits the growth of most Gram-positive bacteria. EMB is often used to confirm the presence of coliforms in a sample. It contains two dyes, eosin and methylene blue in the ratio of 6:1. EMB is a differential microbiological media, which inhibits the growth of Gram-positive bacteria and differentiates bacteria that ferment lactose (e.g., E. coli) from those that do not (e.g., Salmonella, Shigella).[2] Organisms that ferment lactose appear dark/black or green often with "nucleated colonies"—colonies with dark centers.[3] Organisms that do not ferment lactose will appear pink and often mucoid.

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E. coli on EMB agar

This culture media is important in medical laboratories by allowing the identification of enteric bacteria microbes in a short period of time.[4]

  • Rapid lactose fermentation produces acids, which lower the pH. This encourages dye absorption by the colonies, which are now colored purple-black.
  • Lactose non-fermenters may increase the pH by deamination of proteins. This ensures that the dye is not absorbed. The colonies will be colorless.
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Metallic green sheen of E. coli on EMB agar

On EMB if E. coli is grown it will give a distinctive metallic green sheen (due to the metachromatic properties of the dyes, E. coli movement using flagella, and strong acid end-products of fermentation). Some species of Citrobacter and Enterobacter will also react this way to EMB.[5] This medium has been specifically designed to discourage the growth of Gram-positive bacteria.[6]

EMB contains the following ingredients: peptone, lactose, dipotassium phosphate, eosin Y (dye), methylene blue (dye), and agar.

There are also EMB agars that do not contain lactose.

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References

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