Florida State Road 320

State Road 320 (SR 320) is an east–west route in Levy County connecting Manatee Springs State Park to U.S. Route 19 (US 19), 98, and US 27 Alternate (US 27 Alt.).

State Road 320
SR 320 in red, CR 320 in blue
Route information
Maintained by FDOT
Length	5.784 mi[1] (9.308 km)
Major junctions
West end	Manatee Springs State Park
East end	US 19 / US 27 Alt. / US 98 in Chiefland
Location
Country	United States
State	Florida
Counties	Levy
Highway system
Florida State Highway System Interstate US State Former Pre‑1945 Toll Scenic
← US 319 → SR 326

The state-signed portion runs along streets named Manatee Springs Road, Northwest 115th Street, and Northwest 19th Avenue. The road continues east of US 19/98/27 Alt. as County Road 320 along Northeast 120th Street from its intersection with US 19/98/27 Alt. to CR 339 in Newton.

Major intersections

The entire route is in Levy County.

Location	mi[1]	km	Destinations	Notes
​	0.000	0.000	Manatee Springs State Park
Chiefland	5.516	8.877	CR 341 (Northwest 14th Street)
	5.784	9.308	US 19 / US 27 Alt. / US 98 (SR 55)
1.000 mi = 1.609 km; 1.000 km = 0.621 mi