Hydroxylamine oxidoreductase

Hydroxylamine oxidoreductase (HAO) is an enzyme found in the prokaryotic genus Nitrosomonas. It plays a critically important role in the biogeochemical nitrogen cycle as part of the metabolism of ammonia-oxidizing bacteria.

Hydroxylamine Oxidoreductase
Identifiers
EC no.	1.7.3.4
CAS no.	9075-43-8
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

The substrate is hydroxylamine (${\displaystyle {\ce {NH2OH}}}$), a chemical produced biologically by the enzyme Ammonia monooxygenase. The products of the catalyzed reaction are debated, but recent work shows compelling evidence for the production of nitric oxide.^[1]

Structural studies

Crystallographic methods show that HAO (PDB code: 1FGJ) is a cross-linked trimer of polypeptides containing 24 heme cofactors.^[2][3]

Reactivity

For many decades the enzyme was thought to catalyze the following reaction:^[4]

${\displaystyle {\ce {NH2OH + H2O -> NO2^- + 5 H+ + 4e^-}}}$

Recent work in the field, however, reveals that this enzyme catalyzes an entirely different reaction:^[1]

${\displaystyle {\ce {NH2OH -> NO + 3H+ + 3e^-}}}$

Subsequent oxidation of the nitric oxide to nitrite caused by reaction with oxygen accounts for the reactivity previous described by Hooper et al.

Environmental Impact

Nitric oxide, the product of HAO catalysis, is a potent greenhouse gas.^[5] Additionally, the oxidized product of nitric oxide in the presence of oxygen is nitrite - a common pollutant in agricultural run-off.