Methanogen homoaconitase

Methanogen homoaconitase (EC 4.2.1.114, methanogen HACN) is an enzyme with systematic name (R)-2-hydroxybutane-1,2,4-tricarboxylate hydro-lyase ((1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate-forming).^[1] This enzyme catalyses the following chemical reaction

(R)-2-hydroxybutane-1,2,4-tricarboxylate ${\displaystyle \rightleftharpoons }$ (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate (overall reaction)

(1a) (R)-2-hydroxybutane-1,2,4-tricarboxylate ${\displaystyle \rightleftharpoons }$ (Z)-but-1-ene-1,2,4-tricarboxylate + H₂O

(1b) (Z)-but-1-ene-1,2,4-tricarboxylate + H₂O ${\displaystyle \rightleftharpoons }$ (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate

Methanogen homoaconitase
Identifiers
EC no.	4.2.1.114
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

This enzyme catalyses several reactions in the pathway of coenzyme-B biosynthesis in methanogenic archaea.