Methyl acetate hydrolase

Methyl acetate hydrolase, known also as methylacetate acetohydrolase is a hydrolase enzyme that utilizes H2O to carry out the hydrolysis of methyl acetate (CH₃COOCH₃), a weakly lipophillic and polar compound used often as a solvent. In the species Gordonia sp. (strain TY-5), the enzyme is encoded by gene acMB.^[1] The enzyme catalyzes the following reaction,

methyl acetate + H2O = methanol + acetate + H⁺

Methyl acetate hydrolase
Collage of methyl acetate hydrolases from various bacterium, from clockwise: Gordonia sp., Nocardioides perillae, Rhodospira trueperi, Calidifontibacter indicus.
Identifiers
EC no.	3.1.1.114
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

The enzyme is involved in two major pathways, the butanoate metabolism pathway and the catabolic degradation pathway of propanol.^[2][3][4]