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Micro-incineration
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Micro-incineration or microincineration is a technique to determine the manner and distribution of mineral elements in biological cells, biological tissues and organs.[1] Slide preparation of tissues can be used. Examples include calcium (Ca), potassium (K), sodium (Na), magnesium (Mg), iron (Fe), and silicon (Si). The early known figure who discovered besides describing of this incineration was Raspail, an Frenchman who worked as a chemistry during his discovery in 1833.[1][2]

The organic matter is vaporised by heating. The nature and position of the mineral ash is determined microscopically. Aqueous or cryo-EM fixed tissue materials can also be examined under transmission and scanning electron microscopy (TEM & SEM).
The ashing procedure produces cellular oxidised-residues rich in Na2O, CaO, MgO, Fe2O3, SiO2, Ca(PO4)2, Mg(PO4)2, etc., which are detected by X-ray microanalysis with 2-4 times sensitivity gained after incineration of sample, due to increased mineral concentration and reduced nonspecific background radiation.
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Early discovery of Micro-incineration
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The first known person for discovery of and about a Micro-incineration was entirely to Raspail, who is a founder of the chemical microscopy.[2] In earliest reports of its value from technic was a Frenchman of Raspail, when around in 1833, he ashed the epidermal layers of an onion bulbs. Particularly, siliceous skeleton of various plants family is an object of study for nearly a century, which this particular was investigated by von Mohl in 1861. In 1914, a German botanist named Fritz Netolitzky incinerated of sectioned grains of prehistoric cereal that remains of an aid for is identifications. The current cycle of development and application of micro-incineration process which owes for its impetus was primarily to the late figure was Hans Molisch, on the botanical side and subsequent to A. Policard, in the field of the animal histology.[3]
Plethora, since from their given reviews that is rated as "excellent" on animal and clinical phases, the only of the batonical aspects of the incineration data in accordance from the journal would considered to be in this particular point, with exception of about severals that is strictly and thusly as cytological of investigations.[3][clarification needed]
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