Mycothiol-dependent formaldehyde dehydrogenase

In enzymology, a mycothiol-dependent formaldehyde dehydrogenase (EC 1.1.1.306) is an enzyme that catalyzes the chemical reaction

formaldehyde + mycothiol + NAD⁺ ${\displaystyle \rightleftharpoons }$ S-formylmycothiol + NADH + 2 H⁺

mycothiol-dependent formaldehyde dehydrogenase
Identifiers
EC no.	1.1.1.306
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

The 3 substrates of this enzyme are formaldehyde, mycothiol, and NAD⁺, whereas its 3 products are S-formylmycothiol, NADH, and H⁺.^[1][2][3][4] This enzyme catalyses the following chemical reaction

This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is formaldehyde:NAD+ oxidoreductase (mycothiol-formylating). This enzyme is also called NAD/factor-dependent formaldehyde dehydrogenase or S-(hydroxymethyl)mycothiol dehydrogenase.