NAD+ synthase (glutamine-hydrolysing)

In enzymology, a NAD⁺ synthase (glutamine-hydrolysing) (EC 6.3.5.1) is an enzyme that catalyzes the chemical reaction

ATP + deamido-NAD⁺ + L-glutamine + H₂O ${\displaystyle \rightleftharpoons }$ AMP + diphosphate + NAD⁺ + L-glutamate. In eukaryotes, this enzyme contains a glutaminase domain related to nitrilase.^[1]

NAD+ synthase (glutamine-hydrolyzing)
Glutamine-dependent NAD+ synthetase homooctamer, Human
Identifiers
EC no.	6.3.5.1
CAS no.	37318-70-0
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

The substrates of this enzyme are ATP, deamido-NAD+, L-glutamine, and H₂O, whereas its 4 products are AMP, diphosphate, NAD⁺, and glutamate ^[2]

This enzyme participates in glutamate metabolism and nicotinate and nicotinamide metabolism.

Nomenclature

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds carbon-nitrogen ligases with glutamine as amido-N-donor. The systematic name of this enzyme class is deamido-NAD+:L-glutamine amido-ligase (AMP-forming).